Beamline 8.3.1

Macromolecular Crystallography (TomAlberTron)

Endstation 8.3.1 - Tom-Alber-Tron
Endstation 8.3.1 In-Situ - In-Situ Tray Goniometer

Current status	Operational; open to general users
ALS-U Status	This beamline may temporarily close ~2 months before the ALS-U dark time and will resume operations after a commissioning period. For more information, see ALS-U Beamline Impacts and other ALS-U web pages.
Source	Superbend
Minimum energy (eV)	5,000
Maximum energy (eV)	17,000
Wavelength range (Å)	0.73–2.5
Research areas	Biological Sciences
Technique category	Scattering/Diffraction Protein crystallography (PX) Small-molecule crystallography X-ray diffraction (XRD)
Flux/Brightness	1.0 x 10¹² photons/s measured at 3 x 0.35 milliradian divergence at 11,111 eV with ALS at 500 mA
Resolving power	Water-cooled flat double Si(111) Khozu monochromator: E/ΔE = 7,000 measured with Ge(11,1,1water-cooled silicon bent into parabola 200 nanoradian slope ) analyzer crystal Primary Mirror: flat internallyerror Secondary Mirror: uncooled cyllindrical silicon bent into torroid 2:1 demagnification Both mirrors coated with Pt/Rh
Access modes	Onsite Remote - robotic
Additional notes	Primary Detector: Dectris Pilatus3 S 6M Secondary Detector: Hitachi Vortex EX energy-resolving for MAD and excitation scans
Primary contact(s)	James Holton JMHolton@lbl.gov 510-928-5556 George Meigs GMeigs@lbl.gov 510-520-1423
Beamline website	http://bl831.als.lbl.gov/
Beamline phone number	510-495-2083

Endstation 8.3.1

Tom-Alber-Tron

Status	Operational; open to general users
Research areas	Biological Sciences
Technique category	Scattering/Diffraction Protein crystallography (PX) Small-molecule crystallography X-ray diffraction (XRD)
Spot size	0.08 x 0.06 mm (v x h)
Sample information	Sample temperature adjustable from 100-300K "Cool Hand Luke" Robot accepts all major pin types (Hampton, Yale, SPINE, etc.) and lengths (10-24 mm) and supports "delayed data collection mode" where data collection runs are deferred to the end of the shift. This robot does not work directly with cassettes because hand-mounting of samples in the mini-hutch is faster than any robot.
Additional Notes	User-changeable motorized collimators: 100, 75 50, 30 20 microns diameter Goniometer is fast air-bearing spindle capable of "true" MAD with round-robin inverse beam and wavelength changes Sample Manipulator Huber XYZ stage 1.1 micron rms sphere of confusion

Endstation 8.3.1 In-Situ

In-Situ Tray Goniometer

Status	Operational; limited access
Research areas	Biological Sciences
Technique category	Scattering/Diffraction Protein crystallography (PX) X-ray diffraction (XRD)
Sample information	All SBS compliant crystallization trays supported Thin-walled in-situ ready trays such as in-situ-1 highly recommended Ambient temperature only currently supported