Well-known high-resolution structural methods are often used to characterize antibody structures, but many require specialized sample preparation that may perturb antibody structure. We describe here the relatively new method of hydroxyl radical protein footprinting, a solution-state method that can provide structural and kinetic information on antibodies or antibody–antigen interactions useful for therapeutic antibody design. Read more »
Inhalable COVID-19 Protection via Synthetic Nanobodies
Protein structures obtained in part at the ALS helped researchers to increase the potency of simplified antibodies (nanobodies) designed to neutralize SARS-CoV-2. Stable enough to be used in inhalers or nasal sprays, the nanobodies offer a new option, aside from injected vaccines, for COVID-19 prevention and treatment. Read more »
Footprinting Technique Gives ALS Users New Insights
The x-ray footprinting (XFP) technique developed at ALS Beamline 5.3.1 gives researchers a powerful tool for the study of macromolecular structures and dynamics of proteins and nucleic acids in solution. Read more »
