Bacterial microcompartment proteins can assemble into multiple structures, such as sheets, shells, and intermediates. While this is shown artistically in this figure, we study the differences in the pore dynamics within bacterial microcompartment assemblies to assess potential changes in permeability. Read more »
Time-Resolved SAXS Screen of Small-Molecule Drug Candidates
Time-resolved, high-throughput, small-angle x-ray scattering improved the screening of small-molecule drug candidates, providing insight into how they stimulate structural transitions in protein targets. The work will speed the discovery of treatments designed to activate biomolecular dynamics associated with desired therapeutic outcomes. Read more »
Mechanistic Insight into a Viral-Factory Component
Recent protein-structure studies conducted at the ALS provided mechanistic insights into the function of a protein (σNS) involved in viral replication. Understanding these mechanisms will foster the development of therapeutic strategies against viruses that use σNS-like proteins to replicate. Read more »
Symbiotic Nitrogen-Fixing Microbe Evolves into Organelle
Researchers found that a symbiont capable of fixing nitrogen (turning it into a biologically usable form) has evolved into an organelle—an intrinsic part of the algae cells that host it. The discovery is of great interest for understanding organelle genesis and for efforts to engineer agricultural plants with built-in nitrogen-fixing capabilities. Read more »
Scientists Discover First Nitrogen-Fixing Organelle
In two recent papers, an international team of scientists describe the first known nitrogen-fixing organelle within a eukaryotic cell. The organelle is the fourth example in history of primary endosymbiosis—the process by which a prokaryotic cell is engulfed by a eukaryotic cell and evolves beyond symbiosis into an organelle. Read more »
Nitrogen-fixing organelle in a marine alga
A nitrogen-fixing organelle, or “nitroplast,” has been identified in a marine alga on the basis of intracellular imaging and proteomic evidence. This discovery sheds light on the evolutionary transition from endosymbiont to organelle. The image depicts the cell architecture and synchronized cell division of the alga Braarudosphaera bigelowii with nitroplast UCYN-A (large brown spheres). Read more »
Shedding Light on Sea Creatures’ Secrets
Exactly how does coral make its skeleton, a sea urchin grow a spine, or an abalone form the mother-of-pearl in its shell? A new study at the ALS revealed that this process of biomineralization, which sea creatures use to lock carbon away in their bodies, is more complex and diverse than previously thought. Read more »
Clarifying the FLASH Effect for Cancer Radiation Therapy
To clarify the underlying mechanisms of the FLASH effect, in which the delivery of ultrafast, high-intensity doses of radiation to tumors counterintuitively reduces damage to surrounding healthy cells, researchers directly compared the oxidative effects of conventional and FLASH techniques using x-ray footprinting at the ALS. Read more »
Novel modifications of PARP inhibitor veliparib increase PARP1 binding to DNA breaks
The catalytic activity of PARP1 is associated with DNA damage detection and repair among other cellular functions. We describe efforts to modify the allosteric properties of veliparib, a potent catalytic inhibitor of PARP1. These compounds highlight a unique way to trigger PARP1 retention on DNA breaks and open a path to unveil the pharmacological benefits of such inhibitors with novel properties. Read more »
Bifurcation of High- and Low-Energy Electrons in Microbial Metabolism
A class of chemical reaction found only in biology, electron bifurcation channels two electrons from one donor to two separate acceptors, with one electron elevated in energy at the expense of lowering the energy of the second. Researchers used the ALS to study this process in a microbial protein involved in this bioenergetic pathway. Read more »
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